The Staff Research Associate (SRA) assists the principal investigator (PI) with active experiments and the daily tasks related to the operation of a biomedical research laboratory. This is a great opportunity for individuals interested in graduate school in the biomedical sciences.
Our research is primarily focused on: 1) chronic hypoxia sensing and adaptation, 2) mechanisms of pericarditis, and 3) lipid metabolism in hypoxia. We seek to understand how cells, tissues, and organisms respond and adapt to chronic hypoxia at the molecular and whole-body physiology levels. We utilize a variety of in vitro (cell) models and mouse models to investigate specific transcription factors, gene and protein regulatory pathways, and metabolic changes in mediating responses under hypoxia. This work lies at the intersection of hypoxia signaling, lipid metabolism, and cardiovascular disease. We have developed models of cardiovascular disease that are affected by chronic hypoxia, including pulmonary hypertension, heart failure, and obesity. We use various biochemical assays, “multi-omics,” and computational approaches to carefully dissect mechanisms of hypoxia adaptation and lipid rewiring in hypoxia. In addition, we are interested in dissecting mechanisms of recurrent pericarditis, using mouse models of pericarditis and patient samples.
The SRA will have many duties relevant to these goals and it is expected that they will become increasing independent over time. It is expected that the SRA will become well-versed in multiple molecular and biochemical techniques to analyze tissues and cells, such as western blotting, qPCR, single nuclear RNA sequencing, and generation of DNA constructs. They will also assist with various mouse model experiments and take an active role in mouse colony maintenance and lab organization and lab management.
Essential Function (Yes/No)
(To be completed by Supervisor)
10
YesGeneral mouse husbandry and mouse colony maintenance (including breeding, tagging of animals, monitoring mice in different O2 tensions, and PCR genotyping).
50
YesStandard biochemistry and molecular biology techniques including protein isolation (nuclear and cytosolic extracts), western blotting, RNA/DNA isolation, quantitative RT-PCR, PCR, primer generation, agarose gel electrophoresis, DNA cloning, chromatin immunoprecipitation.
15
YesIn vivo mouse experiments, including exposing mice to different O2 tensions, monitoring weights, collecting serum, measuring glucose levels, paired feeding protocols, treating mice with various compounds and small molecules, monitoring activity levels and strength
5
YesStandard data analysis techniques including an ability to manipulate data in spreadsheet format and generate plots, charts, tables, and presentations. Basic bioinformatics of data sets (for example using R, Python, or Matlab)
15
YesStandard tissue culture techniques including propagation of mammalian cell lines, transient and stable transfection of cultured cells, genetic manipulation of cultured cells, treatment of cells with various compounds, exposure to different O2 tensions, isolation of DNA/RNA/protein from cultured cells, and cell viability assays.
5
YesOrdering reagents, organizing laboratory inventory, maintaining antibody and reagent databases, and other various administrative tasks. Preparation of data slides and experiment updates.
100%
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SRA 1
SRA 2 NEX
Additional requirements;
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences. We bring together the world’s leading experts in nearly every area of health. We are home to five Nobel laureates who have advanced the understanding of cancer, neurodegenerative diseases, aging and stem cells.
UCSF is a diverse community made of people with many skills and talents. We seek candidates whose work experience or community service has prepared them to contribute to our commitment to professionalism, respect, integrity, diversity and excellence – also known as our PRIDE values.
In addition to our PRIDE values, UCSF is committed to equity – both in how we deliver care as well as our workforce. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care. Additional information about UCSF is available here.
Join us to find a rewarding career contributing to improving healthcare worldwide.
The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.
The final salary and offer components are subject to additional approvals based on UC policy.
Your placement within the salary range is dependent on a number of factors including your work experience and internal equity within this position classification at UCSF. For positions that are represented by a labor union, placement within the salary range will be guided by the rules in the collective bargaining agreement.
To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html
