Job Description

Status

Full-Time

Standard Hours per Week

40

Job Posting Category

Research

Job Posting Description

Department Summary:
Research Assistant II and Lab Manager for the Laboratory of Joseph Majzoub, MD, Division of Endocrinology, Department of Pediatrics, Boston Children’s Hospital.

Key Responsibilities:

• Maintain multiple mouse colonies including breeding, genotyping, with accurate records. This is one of the most important roles
• Maintain records of experimental methodologies and results in a log of work performed (Lab Archives online notebook).
• Work with PI and independently to design and perform experiments
• Perform a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and analyzing outcomes for completeness and accuracy.
  • Mouse necropsy and tissue processing
  • Mouse tissue histological analysis using light microscopy, immunofluorescence, RNA in situ hybridization (RNAscope)
  • ELISA, RIA, qRTPCR
  • Cell culture ideal but not required
• Maintain equipment, lab area and supplies in a neat manner.
• Process orders for supplies and animals
• Assist PI to write and amend animal protocols
• Collaborate with other members of the Majzoub lab and with other laboratories in the Endocrinology department

Minimum Qualifications
Education

• Bachelor’s degree in biology, neuroscience, or related field

Experience:

• At least 1 year experience managing laboratory mouse colonies
• Preferred experience with PCR, ELISA, RIA, immunocytochemistry, RNAscope
• Good problem-solving skills and independent work ability
• Excellent organizational and communication skills

Office/Site Location

Boston

Regular, Temporary, Per Diem

Regular

Remote Eligibility

Onsite Only

Pay Range

$44657.60-$69773.60 Annual